This protocol describes gating methods to check T cells in mouse thymus, spleen, and blood. We defined different T mobile populations according to extracellular and intracellular markers. Discard the supernatant, resuspend cell pellets with 200 μL 1× D-PBS and Stick to the very same treatment of extracellular staining with antibodies https://kaiserlichesinterieur.de/2024/04/29/entdecken-sie-die-kraft-der-liebe-6-moglichkeiten-mehr-liebe-in-ihrem-leben-zu-kultivieren/